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Determination of Chloramphenicol in Honey and Milk by HPLC Coupled with Aptamer‐Functionalized Fe 3 O 4 /Graphene Oxide Magnetic Solid‐Phase Extraction
Author(s) -
Tu Chunyan,
Guo Yinan,
Dai Yuanyuan,
Wei Wei,
Wang Weiping,
Wu Liang,
Wang Aijun
Publication year - 2019
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14955
Subject(s) - chromatography , extraction (chemistry) , solid phase extraction , detection limit , graphene , high performance liquid chromatography , chloramphenicol , chemistry , elution , aptamer , analyte , solvent , oxide , materials science , organic chemistry , nanotechnology , biochemistry , biology , genetics , antibiotics
An aptamer‐functionalized Fe 3 O 4 /graphene oxide was synthesized by chemical co‐precipitation method and then employed in the magnetic solid‐phase extraction for selective enrichment of chloramphenicol before HPLC. The aptamer was covalently bonded to the Fe 3 O 4 /graphene oxide complex by 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide hydrochloride and N ‐hydroxysuccinimide. Parameters affecting extraction efficiency including solution pH, extraction time and temperature, types and volume of elution solvent, and elution time were investigated in detail. Under the optimal conditions, good linearity was obtained between the peak area and analyte concentration in the range of 7.0 to 1.0 × 10 3 µg/L with the correlation coefficient of 0.9994. The limit of detection and quantitation were 0.24 µg/L and 0.79 µg/L, respectively. The developed method was employed to the analysis of chloramphenicol in honey and milk samples. The recoveries ranged from 80.5% to 105.0% with relative standard deviations less than 8.9%. Practical Application An aptamer‐functionalized Fe 3 O 4 /graphene oxide was synthesized and employed in magnetic solid phase extraction for the enrichment of chloramphenicol before HPLC. The presented assay was employed for the determination of chloramphenicol in honey and milk with satisfactory results.