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Comparison of Lipids Extracted by Different Methods from Chinese Mitten Crab ( Eriocheir sinensis ) Hepatopancreas
Author(s) -
Wang Fengya,
Lin Wei,
Lv Shun,
Jiang Shaotong,
Lin Lin,
Lu Jianfeng
Publication year - 2019
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14946
Subject(s) - hepatopancreas , eriocheir , chinese mitten crab , astaxanthin , polyunsaturated fatty acid , food science , biochemistry , extraction (chemistry) , biology , fatty acid , chemistry , chromatography , carotenoid , fishery
The effects of four different extraction methods (Folch, Soxhlet, two‐step, and enzyme‐assisted aqueous extraction) on the yields, lipid class, fatty acids (FAs) composition, minor components (including carotenoid, cholesterol), and thiobarbituric acid reactive substances values of lipids in the hepatopancreas of Chinese mitten crab ( Eriocheir sinensis ) were investigated. The C16:0, C18:1, and C18:2 were identified to be the dominant FAs in crab lipids, and the FAs were present in the form of triglycerides. The Soxhlet and enzyme‐assisted extraction were more suitable for crab lipids extraction, showing higher extraction rates and oxidative stability. Especially, the lipid extracted by enzyme‐assisted extraction has high carotenoids content. The components of crab lipids extracted by enzyme‐assisted aqueous extraction were further identified using untargeted metabolomics methods. The polyunsaturated fatty acid, sterols, amino acids, products of lipid β‐oxidation and ATP degradation, phosphatidyl ethanolamine, and astaxanthin were founded in crab oil. Practical Application The Chinese mitten crab ( Eriocheir sinensis ) is a popular aquatic food in China. The hepatopancreas is the major lipid storage organ of crab, and the distinctive flavor of crab is mainly from it. To compare the different extraction methods on yield, composition and properties of crab lipids can be helpful for lipids production from crab hepatopancreas. Meanwhile, the crab hepatopancreas lipids are rich in polyunsaturated fatty acids and astaxanthin, and have potential to be as a functional component and a crab flavor additive in food industry.