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Monitoring of 49 Pesticides and 17 Mycotoxins in Wine by QuEChERS and UHPLC–MS/MS Analysis
Author(s) -
He Jia,
Zhang Bo,
Zhang Huan,
Hao LanLan,
Ma TengZhen,
Wang Jing,
Han ShunYu
Publication year - 2019
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14695
Subject(s) - quechers , chromatography , formic acid , chemistry , wine , electrospray ionization , selected reaction monitoring , detection limit , analyte , mass spectrometry , tandem mass spectrometry , pesticide residue , pesticide , food science , agronomy , biology
An effective method for the determination of 49 pesticide residues and 17 mycotoxins in wine by a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and ultrahigh‐performance liquid chromatography–tandem mass spectrometry was developed. The target compounds were extracted with 1% (v/v) formic acid–acetonitrile, and no cleanup steps were required. The extracts were separated on a C18 chromatographic column (2.1 mm × 50 mm, 1.7 µm) with acetonitrile and water with 0.2% formic acid solution and ammonium acetate (10 mM) as the mobile phases under gradient elution at a flow rate of 0.2 mL/min. The determination was conducted using electrospray ionization in positive ion mode with multiple reaction monitoring. The analytes were quantified by comparison with matrix‐matched standard solutions. The good linearities were obtained in the range of 0.05 to 500.0 µg/kg, and the correlation coefficients were all greater than 0.9935. The average recoveries of the 66 target compounds ranged from 69% to 119%, and the RSDs were in the range of 1% to 10%. The limits of detection were in the range of 0.05 to 20.0 µg/kg. The method was proved to be rapid, selective, sensitive, and stable, and it has been applied to analysis of 64 wine samples.