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Authentication of Small Berry Fruit in Fruit Products by DNA Barcoding Method
Author(s) -
Wu Yajun,
Li Meige,
Yang Yange,
Jiang Li,
Liu Mingchang,
Wang Bin,
Wang Yingchun
Publication year - 2018
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14177
Subject(s) - berry , dna barcoding , barcode , biology , dried fruit , authentication (law) , food science , microbiology and biotechnology , computational biology , botany , computer science , evolutionary biology , computer security , operating system
Small berry fruit products are gaining an expanded market due to their high nutrition value. However, the authenticity of products is challenged by adulteration and mislabeling. To establish an accurate and robust method for identifying both known and unknown fruit species in small berry fruit products, DNA barcoding technology based on Sanger sequencing was adopted. To overcome the influence of processing conditions on DNA recovery, mini‐barcodes of rbc L and ITS and a medium‐barcode of psb A‐ trn H were applied. To identify ingredients in products containing mixed species, plasmid cloning was applied to separate mixed barcodes. The method established in this paper could detect 1% to 10% target species in mixed fruit juice.