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Effects of Lactobacillus rhamnosus GG and Escherichia coli Nissle 1917 Cell‐Free Supernatants on Modulation of Mucin and Cytokine Secretion on Human Intestinal Epithelial HT29‐MTX Cells
Author(s) -
Wan Murphy LamYim,
Chen Zhijian,
Shah Nagendra P.,
ElNezami Hani
Publication year - 2018
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14168
Subject(s) - lactobacillus rhamnosus , proinflammatory cytokine , mucin , microbiology and biotechnology , cytokine , secretion , biology , tumor necrosis factor alpha , mucin 2 , monocyte , interleukin 8 , probiotic , immunology , inflammation , bacteria , gene expression , biochemistry , genetics , gene
This study examined modulation effects of cell‐free supernatants of two commonly studied probiotic bacteria Lactobacillus rhamnosus GG (LGG) and Escherichia coli Nissle 1917 (EcN) on mucin and cytokine profiles of human intestinal epithelial HT29‐MTX cells. It was found that LGG and EcN supernatants differentially modulated MUC5AC and MUC5B mRNA and protein, and total mucin‐like glycoprotein secretion. Regarding modulation of cytokine profiles, LGG supernatants moderately influenced the secretion of anti‐inflammatory cytokines such as interleukin (IL)‐4, IL‐5, and IL‐10, while those of EcN exerted a broad proinflammatory effect to intestinal epithelial cells by inducing the secretion of proinflammatory cytokines such as IL‐8, monocyte chemotactic protein‐1, transforming growth factor α, tumor necrosis factor α, granulocyte macrophage colony‐stimulating factor, and interferon γ. These results suggested that LGG and EcN might produce different bioactive products that display differential modulation of mucin and cytokines, which may contribute to intestinal health and/or defense against bacteria/pathogens. Practical Application The results suggested that LGG and EcN might produce different bioactive products that display differential modulation of mucin and cytokines, which may contribute to intestinal health and/or defense against bacteria/pathogens.