z-logo
Premium
Aqueous and Ethanol Extracts of Daylily Flower ( Hemerocallis fulva L.) Protect HUVE Cells Against High Glucose
Author(s) -
Wu WenTzu,
Mong Meichin,
Yang Yachen,
Wang Zhihong,
Yin Meichin
Publication year - 2018
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14137
Subject(s) - glutathione , antioxidant , catalase , reactive oxygen species , glutathione reductase , chemistry , oxidative stress , glutathione peroxidase , apoptosis , prostaglandin e2 , biochemistry , food science , biology , enzyme , endocrinology
The content of several phenolic acids and flavonoids in aqueous extract (AE) and ethanol extract (EE) of daylily flower ( Hemerocallis fulva L.) was analyzed. The effects of AE or EE at 0.5%, 1%, or 2% in HUVE cells against high glucose‐induced cell death, oxidative, and inflammatory damage were examined. Results showed that seven phenolic acids and seven flavonoids could be detected in AE or EE, in the range of 29 to 205 and 41 to 273 mg/100 g, respectively. Compared with the control groups, high glucose raised the activity of caspase‐3 and caspase‐8; suppressed Bcl‐2 mRNA expression and increased Bax mRNA expression; and induced HUVE cells apoptosis. The pretreatments from AE or EE at 1% or 2% reduced caspase‐3 activity and Bax mRNA expression, and enhanced cell viability. High glucose decreased glutathione content; stimulated the production of reactive oxygen species, interleukin‐6, tumor necrosis factor‐alpha, and prostaglandin E 2 ; raised the activity of cyclooxygenase‐2 and nuclear factor kappa B p50/65 binding; and reduced the activity of glutathione peroxidase, glutathione reductase, and catalase in HUVE cells. AE pretreatments at 1% and 2% reversed these changes. These novel findings suggested that daylily flower was rich in phytochemicals, and could be viewed as a potent functional food against diabetes.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here