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Fast and Quantitative Analysis of Ediphenphos Residue in Rice Using Surface‐Enhanced Raman Spectroscopy
Author(s) -
Weng Shizhuang,
Wang Fang,
Dong Ronglu,
Qiu Mengqing,
Zhao Jinling,
Huang Linsheng,
Zhang Dongyan
Publication year - 2018
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14103
Subject(s) - raman spectroscopy , residue (chemistry) , partial least squares regression , analytical chemistry (journal) , surface enhanced raman spectroscopy , spectrometer , materials science , residual , coefficient of determination , chemistry , biological system , chromatography , mathematics , raman scattering , optics , statistics , algorithm , physics , biochemistry , biology
Detection of residual farm chemicals in agricultural crops is a hot topic in the field of food safety. In this study, ediphenphos residue in rice was detected using surface‐enhanced Raman spectroscopy (SERS) on a portable Raman spectrometer. A simple pretreatment method for rice samples was developed, and uniform gold nanorods were used for SERS measurement. Characteristic signals can still be detected when ediphenphos concentration in rice extraction solution was higher than or equal to 0.1 mg/L. Quantitative analysis of ediphenphos was conducted by regression models developed using partial least‐squares regression, random forest and kernel principal component analysis, and root‐mean‐square error of cross validation, coefficient of determination and relative predicted deviation of optimal model were 0.022 mg/L, 0.9967 and 297.45, which indicated the proposed method can predict ediphenphos concentration with high precision. To validate the feasibility of practical application further, rice samples spiked with 10, 5, 1, 0.5, and 0.1 μg/g ediphenphos residue were analyzed using the above method. The predicted recovery was in the range of 93.4% to 102%, and the predicted error was small for residue of each concentration. These results demonstrated that the presented method could be used for accurate and quantitative detection of ediphenphos residue in rice. Practical Application This study developed a surface‐enhanced Raman spectroscopy (SERS) method for detection of ediphenphos in rice coupled with simple extraction protocol and gold nanorods on a portable Raman spectrometer. SERS is a rapid and accurate method which can be applied in agricultural grain safety inspection.