Premium
The Hepatoprotective Effect of Selenium‐Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride‐Induced Chronic Liver Injury in Rats
Author(s) -
Hamid Mohammed,
Abdulrahim Yassin,
Liu Dandan,
Qian Gang,
Khan Alamzeb,
Huang Kehe
Publication year - 2018
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14030
Subject(s) - chemistry , hepatoprotection , aspartate transaminase , glutathione peroxidase , superoxide dismutase , carbon tetrachloride , lipid peroxidation , oxidative stress , alanine transaminase , ccl4 , pharmacology , liver injury , antioxidant , glutathione , endocrinology , medicine , biochemistry , alkaline phosphatase , enzyme , organic chemistry
Abstract The antioxidant and anti‐inflammatory effects of selenium‐enriched yeast (SY) and Gum Arabic (GA) have been reported. This study aimed to determine the hepatoprotective effect of SY and GA combination on carbon tetrachloride (CCl 4 )‐induced chronic liver injury in rats and to explore their synergistic mechanisms of action. Forty adult male Wistar rats randomly allotted to 5 groups: (A) worked as control, (B) was administered CCl 4 , (C‐E) were fed daily by GA, SY, and GA+SY respectively after mixing with basal diet, following CCl 4 ‐intoxication. GA and SY combination significantly ameliorated CCl 4 ‐induced reduction in serum total protein with elevation in aspartate transaminase (AST) and alanine transaminase (ALT) in addition to restoring the histopathological changes and hepatic content of hydroxyproline. GA and SY combination was also effective in reducing lipid peroxidation (MDA), consistent with an increase in total antioxidant capacity (T‐AOC), glutathione (GSH), superoxide dismutase (SOD) activities, indicating the suppression of liver oxidative stress. Furthermore, liver inflammation was ameliorated by GA and SY combination through inhibition of nuclear factor‐kappa (NF‐κB), tumor necrosis factor‐alpha (TNF‐α), cyclooxygenase‐2(COX‐2), monocyte chemotactic protein‐1 (MCP‐1), and toll‐like receptor 4(TLR‐4) over expression in the liver. Moreover, the up‐regulation of proliferating cell nuclear antigen (PCNA) expression by GA and SY combination enhanced the regeneration of liver tissue after CCl 4 ‐administration. The expression of Collagen1, alpha‐smooth muscle actin (α‐SMA), and transforming growth factor‐beta1 (TGFβ1), was obviously ameliorated by GA and SY combination, suggesting the amelioration of profibrotic response of the liver. Taken together, our current study suggests that GA and SY combination exhibit a significant hepatoprotective activity, which more efficient than GA or SY alone. Practical Application Chronic liver diseases are the serious health problems, which increase the morbidity and mortality in the world today. Selenium‐enriched yeast (SY) and Gum Arabic (GA) combination might be potential dietary agents could obviously ameliorate chronic liver damage, higher than GA and SY alone. They act to suppress the inflammation and inhibit the profibrotic response as well as support the liver regeneration.