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Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis , and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor
Author(s) -
Zhang Xiaoguang,
Tsuji Sachiko,
Kitaoka Hayato,
Kobayashi Hiroshi,
Tamai Mitsuru,
Honjoh Kenichi,
Miyamoto Takahisa
Publication year - 2017
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13843
Subject(s) - listeria monocytogenes , salmonella enteritidis , salmonella , escherichia coli , microbiology and biotechnology , biosensor , foodborne pathogen , food microbiology , biology , food science , chemistry , bacteria , biochemistry , genetics , gene
Abstract Detection of foodborne pathogens at very low levels is still a challenge. A custom‐built multichannel surface plasmon resonance (SPR) biosensor and simultaneous enrichment broth (SEB) were used to develop a simultaneous detection method for 3 important foodborne pathogens, Escherichia coli O157:H7 (O157:H7), Salmonella enteritidis , and Listeria monocytogenes , at a very low level. These 3 foodborne pathogens at a very low level (14, 6, and 28 CFU/25 g (mL) for O157:H7, S. enteritidis , and L. monocytogenes , respectively) were inoculated in SEB and incubated at 37 ˚C for 24 h. Sample prepared from the simultaneous enrichment culture was analyzed using the multichannel SPR biosensor and sensor chip immobilized with polyclonal antibodies specific to each of the target pathogens. O157:H7, S. enteritidis , and L. monocytogenes in chicken were detected simultaneously at an inoculum dose of 14, 6, and 28 CFU/25 g, respectively. Our method using a custom‐built multichannel SPR biosensor and enrichment in SEB is expected as a rapid and simultaneous detection method for low levels of O157:H7, S. enteritidis , and L. monocytogenes in food. Practical Application Our method is expected as a rapid and simultaneous detection method for pathogens at very low levels. It has great potential for safety control of food and microbiological detection applications.