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Enrichment and Cytotoxic Activity of Curcuminoids from Turmeric Using Macroporous Resins
Author(s) -
Chen Cheng,
Jin Shuna,
Xiang Xingliang,
Wang Xuanyuan,
Shi Qingxin,
Yang Min,
Ji Simin,
Huang Rongzeng,
Song Chengwu
Publication year - 2017
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13800
Subject(s) - chemistry , chromatography , hela , desorption , adsorption , raw material , mass spectrometry , organic chemistry , in vitro , biochemistry
Curcuminoids are functional secondary metabolites abundant in turmeric. In the present study, a simple and efficient method for enrichment of curcuminoids from turmeric was developed using macroporous resin. Eight different types of macroporous resins were examined by static adsorption/desorption properties and the type of XDA‐7 was selected as the optimum one. Under the optimized conditions, the final contents of refined extract excluded 84.2% of impurities, by comparison with crude extract in a scale‐up experiment. Meanwhile, 8 representative curcuminoids including 4 dihydro‐ and tetrahydro‐curcuminoids were enriched, isolated, and identified by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) and nuclear magnetic resonance (NMR) data. In addition, the individual curcuminoids were prepared to evaluate their cytotoxic activity toward HeLa tumor cell lines. All compounds, especially the trace amount of curcuminoids, demonstrated notable cytotoxic activity. The results supported that those trace amount of curcuminoids can be good candidates for drug development as anticancer agents. The purification process was simple and efficient, which could afford a potential method to enrich and concentrate not only the major curcuminoids, but also the trace amount of ones from turmeric raw materials for industrial applications.