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A Cherry Seed‐Derived Spice, Mahleb, is Recognized by Anti‐Almond Antibodies Including Almond‐Allergic Patient IgE
Author(s) -
Noble Kyle A.,
Liu Changqi,
Sathe Shridhar K.,
Roux Kenneth H.
Publication year - 2017
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13757
Subject(s) - prunus dulcis , polyclonal antibodies , cross reactivity , immunoglobulin e , prunus , rosaceae , western blot , cross reactions , allergy , biology , antibody , botany , immunology , horticulture , biochemistry , cultivar , gene
There are a number of examples of immunologic cross‐reactivity elicited by pollens, fruits, seeds, and nuts of closely related plant species. Such cross‐reactivity is of particular concern for patients with food allergies. In this report, we investigated a spice (mahleb) that is prepared from the kernel of the St. Lucie cherry, Prunus mahaleb , for cross‐reactivity with almond ( Prunus dulcis ), using enzyme‐linked immunosorbent assay (ELISA) and Western blot. Almond and mahleb are members of the same genus. Cross‐reactivity between the mahleb and almond was demonstrated by reaction of cherry and almond kernel protein extracts with antibodies raised against almond proteins. Almond‐specific murine monoclonal IgG, rabbit polyclonal IgG, and almond‐allergic serum IgE each exhibited cross‐reactivity with cherry kernel protein. Because of the demonstrated cross‐reactivity between almond and mahleb, these findings should be of special concern to almond‐allergic patients and attending medical personnel.