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Apoptotic Activity of Lactobacillus plantarum DGK‐17‐Fermented Soybean Seed Extract in Human Colon Cancer Cells via ROS–JNK Signaling Pathway
Author(s) -
Khan Imran,
Kang Sun Chul
Publication year - 2017
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13732
Subject(s) - apoptosis , lactobacillus plantarum , programmed cell death , oxidative stress , cancer cell , fermentation , antioxidant , lactate dehydrogenase , colorectal cancer , downregulation and upregulation , cell growth , biology , chemistry , microbiology and biotechnology , biochemistry , cancer , lactic acid , bacteria , enzyme , gene , genetics
Fermented food has been always possesses upper hand compared to normal food due to its antibacterial, antioxidant, and anticancer properties. Soybeans, which have high nutritional value, are widely consumed in Korea. In this study, soybean seed powder fermented with Lactobacillus plantarum DGK‐17, which was previously isolated from kimchi, showed anticancer potential. Fermented soybean extract (FSE) resulted in morphological changes, reduction of cancer cell colony formation and apoptotic cell death of HCT‐116 colon cancer cells in a dose‐dependent manner, and IC 50 value of 111 μg. FSE treatment caused reduction of cell growth in a dose‐dependent manner via release of lactate dehydrogenase. FSE treatment induced HCT‐116 apoptotic cell death as confirmed by the presence of fragmented nuclei, oxidative burst, and reduced mitochondrial membrane potential (Δ Ψ m ). Further, FSE treatment sensitized cells to ER stress via IRE1‐α induction. FSE treatment also resulted in JNK activation, subsequently causing activation of Bax and downregulation of BCl2. Weakened mitochondrial membrane potential (Δ Ψ m ) also caused release of Cyto C, further activating caspase‐mediated cell death. Therefore, this study reveals the apoptotic role of DGK‐17‐fermented soybean seed extract in human colon cancer HCT‐116 cells.