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In Vitro Digestion and Fermentation of Microencapsulated Tributyrin for the Delivery of Butyrate
Author(s) -
Donovan Joseph D.,
Bauer Laura,
Fahey George C.,
Lee Youngsoo
Publication year - 2017
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13725
Subject(s) - butyrate , tributyrin , fermentation , chemistry , food science , gastrointestinal tract , digestion (alchemy) , large intestine , in vitro , biochemistry , chromatography , enzyme , lipase
Abstract Butyrate possesses negative sensory qualities and is most effectively utilized in the intestine to provide energy to the colonocyte for the maintenance of intestinal health. Butyrate has also shown promise in the treatment of intestinal disorders and diseases such as short bowel syndrome, inflammatory bowel disease, and colon cancer. To modify sensory properties, intestinal release, and butyrate production capabilities, tributyrin (TB) was microencapsulated in whey protein isolate (WPI)‐based and gamma‐cyclodextrin (GC)‐based materials. Using an in vitro digestion and fermentation model, microcapsules containing TB were monitored for their release and production of butyrate in vitro . All samples containing TB showed limited butyrate release (<5%) during oral and gastric stages. In the small intestinal phase, all microcapsules containing TB released approximately 75% of their total butyrate with no significant differences ( P > 0.05) across formulations. During the fermentation phase, GC‐based microcapsules produced significantly more butyrate ( P < 0.001) on a molar basis than all WPI‐based microcapsules. Butyrate production increased significantly ( P < 0.001) over each time interval with GC‐based microcapsules having the highest during the 12 h of fermentation. The GC‐based TB encapsulation systems were able to effectively deliver butyrate to the small intestine and generate butyrate in the large intestine. These microcapsules may, therefore, be beneficial for the maintenance of intestinal health and improvement of disease states across all areas of the gastrointestinal tract.