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Physiological Changes of Surface Membrane in Lactobacillus with Prebiotics
Author(s) -
Pan Mingfang,
Kumaree Kishore K.,
Shah Nagendra P.
Publication year - 2017
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13608
Subject(s) - synbiotics , lactobacillus paracasei , cellobiose , food science , lactobacillus plantarum , inulin , chemistry , lactobacillus , biochemistry , bacteria , probiotic , biology , fermentation , lactic acid , hydrolysis , genetics , cellulase
Abstract Synbiotics are always considered to be beneficial in healthy manipulation of gut environment; however, the purpose of this research was to investigate the dominance of synbiotic over the individual potential of probiotics and prebiotics. Four different types of prebiotics, fructo‐oligosaccharides, raffinose, inulin, and cellobiose, were evaluated based on their varying degree of polymerization, combined each with 2 different Lactobacilli strains, including Lactobacillus paracasei 276 and Lactobacillus plantarum WCFS1. The effects of synbiotics combination on the surface structure were evaluated by analyzing auto‐aggregation, membrane hydrophobicity, and adhesion to Caco‐2 cells. Our results showed that both Lactobacilli exhibited significantly greater degree of attachment to Caco‐2 cells (23.31% and 16.85%, respectively) when using cellobiose as a substrate than with other prebiotics ( P < 0.05). Intestinal adhesion ability was in correlation with the percent of auto‐aggregation, both Lactobacillus exhibited higher percent of auto‐aggregation in cellobiose compared to other prebiotics. These behavioral changes in terms of attachment and auto‐aggregation were further supported with the changes noticed from infrared spectra (FT‐IR).