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Dietary Tryptophan Enhanced the Expression of Tight Junction Protein ZO‐1 in Intestine
Author(s) -
Liu Wenhui,
Mi Shumei,
Ruan Zheng,
Li Jing,
Shu Xugang,
Yao Kang,
Jiang Min,
Deng Zeyuan
Publication year - 2017
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13603
Subject(s) - jejunum , tryptophan , basal (medicine) , barrier function , medicine , tight junction , endocrinology , immunohistochemistry , intestinal mucosa , biology , chemistry , amino acid , biochemistry , microbiology and biotechnology , insulin
Dietary amino acids provide various beneficial effects for our health. The aim of the present study was to assess the effects of tryptophan (Trp) supplementation on barrier function. Ninety‐six healthy finishing pigs (initial body weight 51.49 ± 1.12 kg) were randomly allocated into 2 treatment groups, control group, and 0.2% Trp group. The control group was fed the basal diet, and 0.2% Trp group was fed basal diet plus 0.2% Trp. The trial period is 60 d. Compared with control group, the mRNA abundance of claudin‐3 and zonula occluden‐1 (ZO‐1) in the jejunum in 0.2% Trp group ( P < 0.05) was increased. According to immunohistochemistry and immunoblotting test, the expression of ZO‐1 in jejunum in 0.2% Trp group was also significantly increased compared with the control group ( P < 0.05). These results revealed that Trp enhanced the expression of tight junction protein ZO‐1 in the intestine of pig model. Trp may be potential and beneficial dietary functional factor for regulating the intestinal development and inhibiting intestinal aging.