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Quantitative Analysis of 10 Mycotoxins in Wheat Flour by Ultrahigh Performance Liquid Chromatography‐Tandem Mass Spectrometry with a Modified QuEChERS Strategy
Author(s) -
Zhou Qingxin,
Li Fenghua,
Chen Leilei,
Jiang Dafeng
Publication year - 2016
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13524
Subject(s) - quechers , chromatography , chemistry , tandem mass spectrometry , analyte , mycotoxin , mass spectrometry , sample preparation , extraction (chemistry) , solid phase extraction , electrospray , liquid chromatography–mass spectrometry , calibration curve , electrospray ionization , selected reaction monitoring , detection limit , pesticide residue , pesticide , food science , agronomy , biology
Abstract A simple and sensitive analytical method for quantitative analysis of 10 mycotoxins was developed and validated by a combination of modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure with ultrahigh performance liquid chromatography‐tandem mass spectrometry (UHPLC‐MS/MS). Sample preparation involved QuEChERS with dispersive solid phase extraction for clean‐up, and analysis was performed by reversed‐phase UHPLC‐MS/MS using electrospray negative ionization and multiple reaction monitoring. Under optimized conditions, the calibration curves displayed good linear relationships with all coefficients of determinations ( r 2 ) higher than 0.998. The limits of quantification for all target mycotoxins were lower than 7 μg/kg. Trueness and precision for the analytes were 70% to 116% average recoveries and 2% to 13% relative standard deviations (RSDs). The validated method was used to analyze 46 wheat flour samples for the targeted mycotoxins. The method can be used as a rapid and robust tool for screening mycotoxin in cereal products.