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Identification of the Major Components of Buddleja officinalis Extract and Their Metabolites in Rat Urine by UHPLC‐LTQ‐Orbitrap
Author(s) -
Sun Mohan,
Luo Zhiqiang,
Liu Yang,
Yang Ruirui,
Lu Lina,
Yu Guohua,
Ma Xiaoyun,
Liu Aoxue,
Guo Yafang,
Zhao Haiyu
Publication year - 2016
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13435
Subject(s) - orbitrap , chemistry , chromatography , phenylethanoid , officinalis , tandem mass spectrometry , urine , mass spectrometry , glycoside , traditional medicine , biochemistry , stereochemistry , medicine
Buddleja officinalis Maxim, one of the most popular herbal medicines in China, is widely prescribed for curing eye diseases for centuries. In this study, the major components of B. officinalis extract (BOE) and their metabolites in rat urine were detected and identified by ultra‐high‐pressure liquid chromatography coupled with linear ion trap‐orbitrap tandem mass spectrometry (UHPLC‐LTQ‐Orbitrap). A total of 19 compounds, including 8 flavonoids and 11 phenylethanoid glycosides, were confirmed or tentatively identified from BOE. In vivo , 33 components, including 3 prototypes and 30 metabolies, were confirmed or tentatively identified in rat urine samples. The metabolic pathways of different types of compounds were also proposed. This study would effectively narrow the range of potentially bioactive constituents of BOE and shed light to its action mechanism.