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Hurdle Effect of Antimicrobial Activity Achieved by Time Differential Releasing of Nisin and Chitosan Hydrolysates from Bacterial Cellulose
Author(s) -
Hsiao HuiLing,
Lin ShihBin,
Chen LiChen,
Chen HuiHuang
Publication year - 2016
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13295
Subject(s) - nisin , antimicrobial , staphylococcus aureus , chemistry , chitosan , hydrolysate , escherichia coli , minimum inhibitory concentration , microbiology and biotechnology , food science , bacteria , biochemistry , biology , hydrolysis , organic chemistry , genetics , gene
We investigated the combined antimicrobial effect of nisin and chitosan hydrolysates (CHs) by regulating the antimicrobial reaction order of substances due to differential releasing rate from hydroxypropylmethylcellulose‐modified bacterial cellulose (HBC). The minimum inhibitory concentration of nisin against Staphylococcus aureus and that of CHs against Escherichia coli were 6 IU and 200 μg/mL, respectively. Hurdle and additive effects in antimicrobial tests were observed when nisin was used 6 h before CH treatment against S. aureus ; similar effects were observed when CH was used before nisin treatment against E. coli . Simultaneously combined treatment of nisin and CHs exhibited the low antimicrobial effect. HBC was then selected as the carrier for the controlled release of nisin and CHs. A 90% inhibition in the growth of S. aureus and E. coli was achieved when 30 IU‐nisin‐containing HBC and 62.5 μg/mL‐CH‐containing HBC were used simultaneously. The controlled release of nisin and CHs by using HBC minimized the interaction between nisin and CHs as well as increased the number of microbial targets.