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Valorization of Olive Pomace Oil with Enzymatic Synthesis of 2‐Monoacylglycerol
Author(s) -
Keskin Hasene,
Koçak Yanık Derya,
Mucuk Hatice Neval,
Göğüş Fahrettin,
Fadıloğlu Sibel
Publication year - 2016
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13246
Subject(s) - chemistry , yield (engineering) , lipase , pomace , chromatography , monoacylglycerol lipase , candida antarctica , ethanol , triacylglycerol lipase , extraction (chemistry) , oleic acid , enzyme , food science , organic chemistry , biochemistry , materials science , endocannabinoid system , receptor , metallurgy
2‐Monoacylglycerols (2‐MAG) with a high content of oleic acid at sn‐2 position was synthesized by enzymatic ethanolysis of refined olive pomace oil, which is a byproduct of olive oil processing. Six lipases from different microbial sources were used in the synthesis of 2‐MAG. Immobilized lipase from Candida antarctica gave the highest product yield among the selected lipases. Response surface methodology was applied to optimize reaction conditions; time (4 to 10 h), temperature (45 to 60 °C), enzyme load (10 to 18 wt%), and ethanol:oil molar ratio (30:1 to 60:1). The predicted highest 2‐MAG yield (84.83%) was obtained at 45 °C using 10 (wt%) enzyme load and 50:1 ethanol:oil molar ratio for 5 h reaction time. Experiments to confirm the predicted results at optimum conditions presented a 2‐MAG yield of 82.54%. The purification yield (g 2‐MAG extracted/100 g of total product) was 80.10 and 69.00 for solvent extraction and low‐temperature crystallization, respectively. The purity of the synthesized 2‐MAG was found to be higher than 96%.