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Detection of Different DNA Animal Species in Commercial Candy Products
Author(s) -
MuñozColmenero Marta,
Martínez Jose Luis,
Roca Agustín,
GarciaVazquez Eva
Publication year - 2016
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13225
Subject(s) - animal species , hake , dna , food science , biology , species identification , cloning (programming) , anchovy , food products , dna sequencing , polymerase chain reaction , fish <actinopterygii> , chemistry , zoology , fishery , biochemistry , gene , computer science , programming language
Abstract Candy products are consumed all across the world, but there is not much information about their composition. In this study we have used a DNA‐based approach for determining the animal species occurring in 40 commercial candies of different types. We extracted DNA and performed PCR amplification, cloning and sequencing for obtaining species‐informative DNA sequences. Eight species were identified including fish (hake and anchovy) in 22% of the products analyzed. Bovine and porcine were the most abundant appearing in 27 samples each one. Most products contained a mixture of species. Marshmallows (7), jelly‐types, and gummies (20) contained a significantly higher number of species than hard candies (9). We demonstrated the presence of DNA animal species in candy product which allow consumers to make choices and prevent allergic reaction.