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Kinetics of Thermal Inactivation of Peroxidase and Color Degradation of African Cowpea ( Vigna unguiculata ) Leaves
Author(s) -
Wawire Michael,
Oey Indrawati,
Mathooko Francis M.,
Njoroge Charles K.,
Shitanda Douglas,
Hendrickx Marc
Publication year - 2016
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.13168
Subject(s) - vigna , point of delivery , arrhenius equation , chemistry , reaction rate constant , kinetics , peroxidase , degradation (telecommunications) , atmospheric temperature range , activation energy , thermal , arrhenius plot , nuclear chemistry , analytical chemistry (journal) , food science , horticulture , biochemistry , chromatography , thermodynamics , enzyme , biology , telecommunications , physics , quantum mechanics , computer science
Cowpea leaves form an important part of the diet for many Kenyans, and they are normally consumed after a lengthy cooking process leading to the inactivation of peroxidase (POD) that could be used as an indicator for the potential shelf life of the vegetables. However, color degradation can simultaneously occur, leading to poor consumer acceptance of the product. The kinetics of POD in situ thermal (for thermal treatments in the range of 75 to 100 °C/120 min) inactivation showed a biphasic first‐order model, with Arrhenius temperature dependence of the rate constant. The kinetic parameters using a reference temperature ( T ref ) of 80 °C were determined for both the heat‐labile phase ( k ref = 11.52 ± 0.95 × 10 −2 min −1 and E a of 109.67 ± 6.20 kJ/mol) and the heat‐stable isoenzyme fraction ( k ref = 0.29 ± 0.07 × 10 −2 min −1 and E a of 256.93 ± 15.27 kJ/mol). Color degradation (L*, a*, and b* value) during thermal treatment was investigated, in particular as the “a*” value (the value of green color). Thermal degradation (thermal treatments between 55 and 80 °C per 90 min) of the green color of the leaves followed a fractional conversion model and the temperature dependence of the inactivation rate constant can be described using the Arrhenius law. The kinetic parameters using a reference temperature ( T refC = 70 °C) were determined as k refC = 13.53 ± 0.01 × 10 −2 min −1 and E aC = 88.78 ± 3.21 kJ/mol. The results indicate that severe inactivation of POD (as an indicator for improved shelf life of the cooked vegetables) is accompanied by severe color degradation and that conventional cooking methods (typically 10 min/100 °C) lead to a high residual POD activity suggesting a limited shelf life of the cooked vegetables.