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Prevalence of β‐Lactamase Producing Escherichia coli from Retail Meat in Turkey
Author(s) -
Pehlivanlar Önen Sevda,
Aslantaş Özkan,
Şebnem Yılmaz Ebru,
Kürekci Cemil
Publication year - 2015
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12984
Subject(s) - nalidixic acid , biology , escherichia coli , tetracycline , microbiology and biotechnology , trimethoprim , antibiotics , gene , genetics
Extended spectrum β‐lactamase (ESBL) and plasmid‐mediated AmpC β‐lactamase (pAmpC) producing Escherichia coli have been shown to be present in humans and animals representing a significant problem worldwide. This study aimed to search the presence of ESBL and/or AmpC‐producing E. coli in retail meats (chicken and beef) in Turkey. A total of 88 β‐lactamase‐producing E. coli were isolated from chicken ( n = 81/100) and beef meat ( n = 7/100) samples and their susceptibility to several antimicrobials were tested using disc diffusion method. E. coli isolates were further characterized for their phylogenetic groups. β‐Lactamase encoding ( bla TEM , bla SHV , bla OXA , bla CTX‐M , and bla AmpC ) and quinolone resistance genes ( qnrA , qnrB , qnrS , qepA , and acc(6′)‐Ib‐cr ) were also secreened by polymerase chain reaction (PCR). However, in regard to β‐lactamase genes, 84 of 88 isolates were positive for bla CTX‐M‐1 ( n = 39), bla CTX‐M‐3 ( n = 5), bla CTX‐M‐15 ( n = 4), bla TEM‐1b ( n = 2), bla SHV‐12 ( n = 1), bla CTX‐M‐1 / bla TEM‐1b ( n = 10), bla CTX‐M‐1 / bla TEM‐1b / bla SHV‐5 ( n = 1), bla CTX‐M‐1 / bla CMY‐2 ( n = 1) and bla TEM‐1b / bla CMY‐2 ( n = 6), bla CTX‐M‐15 / bla SHV‐12 ( n = 1), bla CTX‐M‐15 / bla TEM‐1b ( n = 1), bla TEM‐1b / bla SHV‐12 ( n = 1) , and bla CMY‐2 ( n = 12) genes. Resistance to cefuroxime (75.6% and 85.7%), nalidixic acid (89% and 85.7%), tetracycline (91.4% and 100%), streptomycin (40.2% and 100%), and trimethoprim‐sulfamethoxazole (36.6% and 85.7%) was observed among strains isolated from chicken and beef, respectively. However, all isolates were found to be susceptible to amikacin, imipenem, and cefepime. Resistance to ampicillin and cefoxitin was significantly linked to bla CMY‐2 gene, while there was a significant correlation between CTX‐M type ESBL and antimicrobial resistance to cefuroxime and streptomycin ( P < 0.05). The results of this study suggest that raw chicken retail meats are highly contaminated with ESBL‐producing E. coli implementing a great risk to human health in Turkey.