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Species Diversity and Pheno‐ and Genotypic Antibiotic Resistance Patterns of Staphylococci Isolated from Retail Ground Meats
Author(s) -
Guran Husnu Sahan,
Kahya Serpil
Publication year - 2015
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12893
Subject(s) - microbiology and biotechnology , ampicillin , tetracycline , penicillin , antibiotic resistance , biology , sccmec , erythromycin , genotype , ofloxacin , veterinary medicine , antibiotics , staphylococcus aureus , ciprofloxacin , medicine , bacteria , methicillin resistant staphylococcus aureus , gene , biochemistry , genetics
The presence and species diversity of staphylococci in 250 ground beef and lamb meat samples obtained from Diyarbakir, Turkey were investigated. The presence of the 16S rRNA gene, mecA , nuc , pvl , and femA was analyzed by multiplex PCR. Pheno‐ and genotypic antibiotic resistance profiles of 208 staphylococci isolates were established. Of the ground beef and ground lamb samples, 86.4% and 62.4% were positive for staphylococci, respectively. Staphylococcus aureus , S. saprophyticus , S. hominis , S. lentus , S. pasteuri , S. warneri , S. intermedius , and S. vitulinus made up 40.8%, 28.8%, 11%, 3.8%, 3.8%, 2.4%, 2.4%, and 2.4% of isolates, respectively. Of the 85 S. aureus isolates, 40%, 47%, and 5.8% carried femA , mecA , and pvl , respectively, whereas the corresponding rates for the 118 coagulase‐negative staphylococci (CoNS) were 0%, 10.1%, and 0%, respectively. We determined from the 208 isolates, the highest antibiotic resistances were to tetracycline and oxytetracycline (85.5%), followed by penicillin (51.4%), novobiocin (45.6%), ampicillin (39.9%), and doxycycline (31.7%), using the Clinical and Laboratory Standards Inst. (CLSI) method. All isolates were sensitive to gentamycin, ofloxacin, and tobramycin, but 2.3% of the S. aureus isolates had resistance to vancomycin. The staphylococci isolates carried tet(K) , blaZ , tet(L) , tet(W) , cat , tet(S) , tet(M) , ermB , ermA , and ermC antibiotic resistance genes at rates of 59%, 51.7%, 36.9%, 31.8%, 27.2%, 27.2%, 24.4%, 18.1%, 7.9%, and 3.9%, respectively.