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Comparison of 3 Selective Media for Enumeration of Bacillus cereus in Several Food Matrixes
Author(s) -
Chon JungWhan,
Song KwangYoung,
Kim Hyunsook,
Seo KunHo
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12594
Subject(s) - bacillus cereus , cereus , agar , food science , mannitol , enumeration , polymyxin , biology , bromothymol blue , brilliant green , microbiology and biotechnology , chemistry , bacteria , chromatography , biochemistry , antibiotics , mathematics , genetics , organic chemistry , combinatorics
In this study, we compared the inclusivity, exclusivity, recoverability, and selectivity of the 3 selective agars (mannitol yolk polymyxin B agar [MYPA], polymyxin pyruvate egg yolk mannitol bromothymol blue agar [PEMBA], and Brillance Bacillus cereus agar [BBC agar]) for Bacillus cereus ( B. cereus ) from pure culture and several food samples. BBC agar showed greater exclusivity and selectivity in pure culture and in foods with high background flora, respectively; however, all the tested media showed similar recoverability ( P > 0.05) of B. cereus in pure culture and in most foods. Our results suggest that BBC agar could be useful to enumerate B. cereus from, in particular, food matrixes with high background competing micro flora.