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The Impact of Ozone Treatment on Changes in Biologically Active Substances of Cardamom Seeds
Author(s) -
Joanna Brodowska Agnieszka,
Śmigielski Krzysztof,
Nowak Agnieszka,
Brodowska Katarzyna,
Catthoor Rik,
Czyżowska Agata
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12591
Subject(s) - polyphenol , chemistry , ozone , antioxidant , food science , human decontamination , antioxidant capacity , composition (language) , control sample , scavenging , microorganism , botany , biochemistry , bacteria , biology , organic chemistry , physics , linguistics , philosophy , nuclear physics , genetics
The overall objective of this study was to develop a decontamination method against microorganisms in cardamom ( Elettaria cardamomum (L.) Maton) seeds using ozone as a decontaminating agent. Ozone treatment was conducted 3 times, at 24‐h intervals, and the parameters of the process were determined assuring the least possible losses of biologically active substances (essential oils and polyphenols): ozone concentration 160 to 165.0 g/m 3 ; flow rate 0.1 L/min; pressure 0.5 atm; time 30 min. After each step of decontamination, the microbiological profile of the cardamom seeds was studied, and the contaminating microflora was identified. Next to the microbiological profile, the total polyphenol content (TPC), composition of essential oils, free radical‐scavenging capacity, total antioxidant capacity, ferric‐reducing antioxidant power (FRAP), and LC–MS polyphenol analysis were determined. This study shows that extract from cardamom seeds after ozone treatment is characterized by a better radical scavenging activity (IC 50 = 24.18 ± 0.04 mg/mL) than the control sample (IC 50 = 31.94 ± 0.05 mg/mL). The extract from cardamom seeds after ozone treatment showed an improved FRAP activity as well (613.64 ± 49.79 mmol TE/g compared to 480.29 ± 30.91 mmol TE/g of control sample). The TPC and the total antioxidant capacity were negatively affected, respectively, 41.2% and 16.2%, compared to the control sample.