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Stability of Iron–Quercetin Complexes in Synthetic Wine under In Vitro Digestion Conditions
Author(s) -
Escudero Leticia B.,
Fusari Cecilia M.,
Altamirano Jorgelina C.,
Camargo Alejandra B.,
Wuilloud Rodolfo G.
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12584
Subject(s) - chemistry , wine , chromatography , polyphenol , quercetin , digestion (alchemy) , atomic absorption spectroscopy , high performance liquid chromatography , metal , dissociation (chemistry) , elution , food science , organic chemistry , antioxidant , physics , quantum mechanics
Wine is a dietary source of polyphenolic compounds with reported health benefits when moderately consumed. Several of these compounds can associate with metals forming complexes. Therefore, this work was conducted to reach a better understanding of the nature and chemical stability of wine‐derived Fe 3+ –quercetin complexes in a digestion model. The stability of the complexes in a synthetic (simulated) wine was studied before and after in vitro gastric and intestinal digestions by high‐performance liquid chromatography (HPLC) with UV‐Vis detection. Metal determination was performed by atomic absorption spectrometry (ETAAS) to evaluate possible dissociation of complexes. During HPLC analysis all peaks eluted from the chromatographic column were collected, acidified, and analyzed by ETAAS. The results showed that complexes remain substantially stable after gastric digestion conditions, with recoveries of 84% to 90%. Although metal complexes were partially degraded during intestinal digestion, 41% to 45% of the Fe 3+ –quercetin complexes was recovered.