Simultaneous Purification of Limonin, Nomilin and Isoobacunoic Acid from Pomelo Fruit ( Citrus grandis ) Segment Membrane | Zendy

Xiang Yu | Zendy; Cao Jinping | Zendy; Luo Fenglei | Zendy; Wang Dengliang | Zendy; Chen Wei | Zendy; Li Xian | Zendy; Sun Chongde | Zendy; Chen Kunsong | Zendy

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Simultaneous Purification of Limonin, Nomilin and Isoobacunoic Acid from Pomelo Fruit ( Citrus grandis ) Segment Membrane

Author(s) -

Xiang Yu,

Cao Jinping,

Luo Fenglei,

Wang Dengliang,

Chen Wei,

Li Xian,

Sun Chongde,

Chen Kunsong

Publication year - 2014

Publication title -

journal of food science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 150

eISSN - 1750-3841

pISSN - 0022-1147

DOI - 10.1111/1750-3841.12581

Subject(s) - limonin , naringin , chromatography , chemistry , high performance liquid chromatography , sephadex , biochemistry , enzyme

A method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo ( Citrus Grandis ). This method includes 3 steps, removing most impurities by macroporous resin HZ‐816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi‐preparative HPLC. Naringin was partially purified as a by‐product of this process using Sephadex LH‐20. All limonoids purified through this method reached 95% purity. The purified limonin, nomilin and isoobacunoic acid were identified according to the retention time of the standard substances using HPLC and characteristic fragment ions of LC‐MS/MS.

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