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Simultaneous Purification of Limonin, Nomilin and Isoobacunoic Acid from Pomelo Fruit ( Citrus grandis ) Segment Membrane
Author(s) -
Xiang Yu,
Cao Jinping,
Luo Fenglei,
Wang Dengliang,
Chen Wei,
Li Xian,
Sun Chongde,
Chen Kunsong
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12581
Subject(s) - limonin , naringin , chromatography , chemistry , high performance liquid chromatography , sephadex , biochemistry , enzyme
A method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo ( Citrus Grandis ). This method includes 3 steps, removing most impurities by macroporous resin HZ‐816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi‐preparative HPLC. Naringin was partially purified as a by‐product of this process using Sephadex LH‐20. All limonoids purified through this method reached 95% purity. The purified limonin, nomilin and isoobacunoic acid were identified according to the retention time of the standard substances using HPLC and characteristic fragment ions of LC‐MS/MS.