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Ultra‐Performance Liquid Chromatographic Determination of L‐Ergothioneine in Commercially Available Classes of Cow Milk
Author(s) -
Sotgia Salvatore,
Pisanu Elisabetta,
Cambedda Debora,
Pintus Gianfranco,
Carru Ciriaco,
Zinellu Angelo
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12564
Subject(s) - chromatography , chemistry , hydrophilic interaction chromatography , detection limit , derivatization , ammonium acetate , high performance liquid chromatography , ammonium , acetonitrile , organic chemistry
A new efficient and sensitive precolumn hydrophilic interaction ultra‐performance liquid chromatography (HILIC‐UPLC) method was established for the quantitative determination of L‐ergothioneine (ERT) in milk. After derivatization of ERT with 7‐diethylamino‐3‐[4‐(iodoacetamido)phenyl]‐4‐methylcoumarin, chromatographic separation was achieved in a fairly short time, less than 5 min, on a 100 × 2.1 mm Waters Cortecs UPLC HILIC 1.6‐μm column, by using a mixture of 30 mmol/L ammonium acetate/acetonitrile (10:90, v/v) as a mobile phase flowing isocratically at 0.9 mL/min. Limit of detection and the limit of quantification were 0.03 and 0.10 μmol/L, respectively. The method exhibited linearity in a concentration range of 0.16 and 5.08 μmol/L. Mean recovery was 106.66%, whereas intra‐ and interassay precisions were determined to be within 6 RSD%. On average, ERT concentration in different commercially available classes of cow milk was found to be 0.442 ± 0.191 μmol/L, with the highest levels in the ultra‐high temperature milks and low values in the unprocessed and HTST whole milks. In this light, our experiments suggest that ERT could be used as a marker for the heat treatment of milk.