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Development of High‐Performance Liquid Chromatographic for Quality and Authenticity Control of Chinese Propolis
Author(s) -
Cuiping Zhang,
Shuai Huang,
Wenting Wei,
Shun Ping,
Xiaoge Shen,
Yajing Li,
Fuliang Hu
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12510
Subject(s) - propolis , pinocembrin , galangin , chemistry , ferulic acid , caffeic acid , vanillic acid , myricetin , chrysin , chromatography , food science , flavonoid , kaempferol , biochemistry , antioxidant
A RP‐high‐performance liquid chromatography (HPLC) method was developed for quality control of Chinese propolis by simultaneous analysis of 12 flavonoids and 8 phenolic acids. The results showed that vanillic acid, rutin, myricetin, and luteolin were not detected in all of the analyzed propolis and poplar tree gum samples. The caffeic acid, ferulic acid and p‐coumaric acid were not detected in poplar tree gum but were detected in propolis, which suggest that they are practical indexes of distinguishing propolis from poplar tree gum. The flavonoid profiles of poplar tree gum were found to be similar to those of propolis, which are dominated by pinobanksin, pinocembrin, 3‐O‐acetylpinobanksin, chrysin, and galangin. Therefore, the proposed method could be applied to exclude poplar tree gum from propolis with cafferic acid, ferulic acid, and p‐coumaric acid as qualitative markers, and distinguish poplar source resin from other illegal substances, and evaluate the quality grading of poplar‐type propolis with pinobanksin, pinocembrin, 3‐O‐acetylpinobanksin, chrysin, and galangin as qualitative and quantitative markers.