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GC‐MS‐Based Metabolite Profiling of Cosmos caudatus Leaves Possessing Alpha‐Glucosidase Inhibitory Activity
Author(s) -
Javadi Neda,
Abas Faridah,
Hamid Azizah Abd,
Simoh Sanimah,
Shaari Khozirah,
Ismail Intan Safinar,
Mediani Ahmed,
Khatib Alfi
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12491
Subject(s) - chemistry , chromatography , gas chromatography–mass spectrometry , alpha glucosidase , metabolite , ethanol , mass spectrometry , food science , biochemistry , enzyme
Cosmos caudatus , which is known as “Ulam Raja,” is an herbal plant used in Malaysia to enhance vitality. This study focused on the evaluation of the α‐glucosidase inhibitory activity of different ethanolic extracts of C. caudatus . Six series of samples extracted with water, 20%, 40%, 60%, 80%, and 100% ethanol (EtOH) were employed. Gas chromatography‐mass spectrometry (GC‐MS) and orthogonal partial least‐squares (OPLS) analysis was used to correlate bioactivity of different extracts to different metabolite profiles of C. caudatus . The obtained OPLS scores indicated a distinct and remarkable separation into 6 clusters, which were indicative of the 6 different ethanol concentrations. GC‐MS can be integrated with multivariate data analysis to identify compounds that inhibit α‐glucosidase activity. In addition, catechin, α‐linolenic acid, α‐D‐glucopyranoside, and vitamin E compounds were identified and indicate the potential α‐glucosidase inhibitory activity of this herb.