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Shiga‐Toxin Genes and Genetic Diversity of Escherichia coli Isolated from Pasteurized Cow Milk in Brazil
Author(s) -
Hoffmann Simone Aparecida,
Pieretti Gabriella Giani,
Fiorini Adriana,
Patussi Eliana Valéria,
Cardoso Rosilene Fressatti,
Mikcha Jane Martha Graton
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12477
Subject(s) - stx2 , escherichia coli , shiga toxin , biology , pasteurization , microbiology and biotechnology , virulence , polymerase chain reaction , intergenic region , genetic diversity , genotype , gene , shiga like toxin , genetics , food science , genome , population , demography , sociology
This study evaluated the genetic similarity and prevalence of the stx1 , stx2 , eae , and ehxA genes in Escherichia coli isolated from pasteurized cow milk. Eighty‐seven E. coli isolates from pasteurized cow milk from 22 dairies located in northwestern Paraná state, Brazil, were analyzed. Genetic similarity was evaluated using enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC‐PCR) and repetitive extragenic palindromic sequence PCR (REP‐PCR). E. coli isolates were also analyzed by PCR to investigate the presence of the stx1 , stx2 , eae , and ehxA genes. ERIC‐PCR and REP‐PCR clustered 87 bacterial isolates in 76 and 81 genomic profiles, respectively. Both techniques revealed high genetic diversity among the E. coli isolates, confirming the possibility of their use in epidemiological studies. The stx1 , stx2 , eae , and ehxA virulence genes were not detected in E. coli isolates, indicating a low prevalence of Shiga toxin‐producing E. coli in milk produced in the region studied.