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Detection of C. Botulinum Types in Honey by mPCR
Author(s) -
Gücükoğlu Ali,
Terzi Göknur,
Çadirci Özgür,
Alişarli Mustafa,
Kevenk Onur,
Uyanik Tolga
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12402
Subject(s) - clostridium botulinum , botulism , apiary , biology , multiplex polymerase chain reaction , food science , veterinary medicine , microbiology and biotechnology , toxin , medicine , honey bees , polymerase chain reaction , botany , gene , biochemistry
The aim of this study was to determine the prevalence of Clostridium botulinum in honey samples using conventional methods and multiplex PCR (mPCR). A total number of 150 honey samples were randomly collected from apiaries, retail shops, weekly open bazaars, and supermarkets in Samsun, Turkey. Of 150 honey samples, 4 (2.6%) were positive for the botulinum neurotoxin gene by mPCR analysis. A total of 4 C. botulinum isolates were obtained from the mPCR positive samples, of which 3 were type A and 1 was type B. No samples were positive regarding the type E and type F neurotoxin genes. This is the first report of type A and type B spores of C. botulinum being detected and isolated in Turkey. This study revealed that some honey samples may present a potential hazard for food borne and infant botulism.