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Evaluation of Potassium‐Clavulanate‐Supplemented Modified Charcoal‐Cefoperazone‐Deoxycholate Agar for Enumeration of Campylobacter in Chicken Carcass Rinse
Author(s) -
Chon JungWhan,
Kim HongSeok,
Kim Hyunsook,
Oh DeogHwan,
Seo KunHo
Publication year - 2014
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12388
Subject(s) - campylobacter , cefoperazone , agar , enumeration , activated charcoal , agar plate , food science , chemistry , microbiology and biotechnology , significant difference , campylobacter jejuni , biology , chromatography , bacteria , antibiotics , medicine , mathematics , genetics , organic chemistry , antibiotic resistance , adsorption , imipenem , combinatorics
Potassium‐clavulanate‐supplemented modified charcoal‐cefoperazone‐deoxycholate agar (C‐mCCDA) that was described in our previous study was compared with original mCCDA for the enumeration of Campylobacter in pure culture and chicken carcass rinse. The quantitative detection of viable Campylobacter cells from a pure culture, plated on C‐mCCDA, is statistically similar ( P > 0.05) to mCCDA. In total, 120 chickens were rinsed using 400 mL buffered peptone water. The rinses were inoculated onto C‐mCCDA and mCCDA followed by incubation at 42 °C for 48 h. There was no statistical difference between C‐mCCDA (45 of 120 plates; mean count, 145.5 CFU/mL) and normal mCCDA (46 of 120 plates; mean count, 160.8 CFU/mL) in the isolation rate and recovery of Campylobacter ( P > 0.05) from chicken carcass rinse. The Pearson correlation coefficient value for the number of Campylobacter cells recovered in the 2 media was 0.942. However, the selectivity was much better on C‐mCCDA than on mCCDA plates ( P < 0.05). Significantly fewer C‐mCCDA plates (33 out of 120 plates; mean count, 1.9 CFU/mL) were contaminated with non‐ Campylobacter cells than the normal mCCDA plates (67 out of 120 plates; mean count, 27.1 CFU/mL). The C‐mCCDA may provide improved results for enumeration of Campylobacter in chicken meat alternative to mCCDA with its increased selectivity the modified agar possess.