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Prevalence of Nitrite and Nitrate Contents and Its Effect on Edible Bird Nest's Color
Author(s) -
Paydar Mohammadjavad,
Wong Yi Li,
Wong Won Fen,
Hamdi Omer Abdalla Ahmed,
Kadir Noraniza Abd.,
Looi Chung Yeng
Publication year - 2013
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12313
Subject(s) - nitrite , nitrate , chemistry , sodium nitrite , nest (protein structural motif) , white (mutation) , food science , hemoglobin , biochemistry , organic chemistry , gene
Edible bird nests (EBNs) are important ethnomedicinal commodity in the Chinese community. Recently, But and others showed that the white EBNs could turn red by vapors from sodium nitrite (NaNO 2 ) in acidic condition or from bird soil, but this color‐changing agent remained elusive. The aim of this study was to determine the prevalence of nitrite and nitrate contents and its affects on EBN's color. EBNs were collected from swiftlet houses or caves in Southeast Asia. White EBNs were exposed to vapor from NaNO 2 in 2% HCl, or bird soil. The levels of nitrite (NO 2 − ) and nitrate (NO 3 − ) in EBNs were determined through ion chromatography analysis. Vapors from NaNO 2 in 2% HCl or bird soil stained white bird nests to brown/red colors, which correlated with increase nitrite and nitrate levels. Moreover, naturally formed cave‐EBNs (darker in color) also contained higher nitrite and nitrate levels compared to white house‐EBNs, suggesting a relationship between nitrite and nitrate with EBN's color. Of note, we detected no presence of hemoglobin in red “blood” nest. Using infrared spectra analysis, we demonstrated that red/brown cave‐EBNs contained higher intensities of C‐N and N‐O bonds compared to white house‐EBNs. Together, our study suggested that the color of EBNs was associated with the prevalence of the nitrite and nitrate contents.