Premium
Czech Ethanol‐Free Propolis Extract Displays Inhibitory Activity against a Broad Spectrum of Bacterial and Fungal Pathogens
Author(s) -
Netíková Ladislava,
Bogusch Petr,
Heneberg Petr
Publication year - 2013
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12230
Subject(s) - propolis , antimicrobial , enterococcus faecalis , microbiology and biotechnology , listeria monocytogenes , candida albicans , staphylococcus aureus , microsporum canis , aspergillus fumigatus , chemistry , biology , food science , bacteria , antifungal , genetics
Propolis acts primarily as a biocide against invasive bacteria and fungi in the hive, suggesting its potential for industrial applications. In food application, propolis is considered as a chemical preservative in meat products, extending shelf life of frozen meat and other food. The mechanism of action is still unclear due to the synergy of multiple compounds contained in propolis and due to parallel targeting of multiple pathways within each affected organism. Here, we examined the antimicrobial properties of dimethylsulfoxide (DMSO) Czech propolis extract. Until recently, DMSO was only rarely used in the propolis studies, although the other solvents tested (mostly ethanol) may significantly affect the observed inhibitory effects, notwithstanding the antimicrobial effects of ethanol itself. Here, we provide results of zone inhibition tests against Aspergillus fumigatus , Microsporum gypseum , Microsporum canis , Candida albicans , Escherichia coli , Staphylococcus aureus , Listeria monocytogenes , and Enterococcus faecalis . Although we determined inhibitory effects against all the microorganisms tested, the dose‐dependent response curves were not similar to each other. While inhibitory effects against C. albicans or S. aureus were strictly dose‐dependent, responses of M. gypseum and E. faecalis displayed plateau across the broad range of concentrations tested. Interestingly, response of E. coli revealed the double‐peak dose‐dependent curve, and responses of M. canis and L. monocytogenes decreased at the highest concentrations tested. Suggested is evaluation of DMSO propolis extracts in experimental treatment of human and veterinary infections, preferably in multitherapy with antibiotics.