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Comparison between Supercritical CO 2 Extraction and Hydrodistillation for Two Species of Eucalyptus: Yield, Chemical Composition, and Antioxidant Activity
Author(s) -
Herzi Najia,
Bouajila Jalloul,
Camy Séverine,
Cazaux Sylvie,
Romdhane Mehrez,
Condoret Jean Stéphane
Publication year - 2013
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.12113
Subject(s) - dpph , chemistry , abts , eucalyptus camaldulensis , gallic acid , extraction (chemistry) , chromatography , gas chromatography , eucalyptus , botany , antioxidant , food science , organic chemistry , biology
In this work, 2 Eucalyptus species extracts ( Eucalyptus cinerea and Eucalyptus camaldulensis) were prepared by hydrodistillation (HD) and supercritical carbon dioxide extraction (SCE) techniques. The best yields of E. cinerea and E. camaldulensis (27.5 and 8.8 g/kg, respectively) were obtained using SCE at 90 bar, 40 °C compared to HD (23 and 6.2 g/kg, respectively). Extracts were quantified by gas chromatography‐flame ionization detection and identified by gas chromatography‐mass spectrometry. 1,8‐cineole and p‐menth‐1‐en‐8‐ol were the major compounds of E. cinerea essential oil obtained by HD (64.89% and 8.15%, respectively) or by SCE (16.1% and 31.87%, respectively). Whereas, in case of E. camaldulensis , 1,8‐cineole (45.71%) and p‐cymene (17.14%) were the major compounds obtained by HD, and 8,14‐cedranoxide (43.79%) and elemol (6.3%) by SCE. Their antioxidant activity was assessed using 2 methods: 2,2‐azino‐di‐3‐ethylbenzothialozine‐sulphonic acid radical cation (ABTS •+ ) and 2,2‐diphenyl‐1‐picrylhydrazyl radical (DPPH • ). In the SCE extracts from both E. cinerea and E. camaldulensis , a promising radical scavenging activity was observed with ABTS •+ , (65 and 128 mg/L, respectively). The total phenolics composition of the extracts was measured and the range was 2 to 60 mg of gallic acid equivalent/g dry plant material. The SCE method was superior to HD, regarding shorter extraction times (30 min for SCE compared with 4 h for HD), a low environmental impact, allows production of nondegraded compounds and being part of green chemistry.