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The optimal dose of Protaetia brevitarsis seulensis (Kolbe) for providing protective effects against CCl 4 ‐induced hepatic damage in rats
Author(s) -
Lee Kwang Yeon,
Bae In Young
Publication year - 2021
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12499
Subject(s) - fibrinogen , prothrombin time , partial thromboplastin time , pharmacology , alanine aminotransferase , necrosis , larva , alanine transaminase , biology , medicine , zoology , chemistry , endocrinology , biochemistry , coagulation , botany
Larvae of edible insects Protaetia brevitarsis seulensis (Kolbe) is well known in Korean traditional medicine for improvement of blood circulation. This study focused on the optimal dose of P. brevitarsis larvae for protection against CCl 4 ‐induced hepatic injury in rats. The optimal dose of P. brevitarsis larvae was estimated by intraperitoneal administration of 100, 300, 1,000, or 3,000 mg/kg, once daily for 5 days, to CCl 4 ‐induced hepatotoxicity rats. CCl 4 administration showed much higher levels of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, compared to control groups. Also, histopathological results showed extensive liver lesions, characterized by substantial necrosis, steatosis, and hemorrhage. In addition, an increased prothrombin time (PT) and activated partial thromboplastin time (aPTT) level and decreased fibrinogen level was observed in the CCl 4 groups. In contrast, the treatment of P. brevitarsis larvae showed significant protection against the CCl 4 ‐induced hepatotoxicity reducing the serum AST and ALT activities ( P < 0.05); the lowest level of serum AST and ALT activities were observed in the group treated with 100 and 300 mg/mL, respectively. Moreover, the treatment with P. brevitarsis larvae at 100 and 300 mg/mL significantly reduced PT and aPTT values, and increased the fibrinogen level, when compared to the CCl 4 ‐treatment alone ( P < 0.05). In intoxicated rats, the doses of P. brevitarsis larvae greater than 300 mg/kg did not enhance its protective action (1,000 mg/mL group) or was not as effective (3,000 mg/mL group). The results indicate that P. brevitarsis larvae revealed a protective effect against CCl 4 acute hepatotoxicity including coagulation disorders and the optimum dose was 300 mg/kg.