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Insecticidal activity of Origanum majorana L. essential oil as anti‐cholinergic agent
Author(s) -
Prabu Sivaprasath,
Jing Dapeng,
Chandran Viswanathan,
Mathew Preethy
Publication year - 2020
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12459
Subject(s) - origanum , biology , aché , essential oil , food science , toxicology , toxicity , comet assay , biochemistry , chemistry , enzyme , acetylcholinesterase , dna damage , organic chemistry , dna
The present study was focused on exploring the presence of active compounds in Origanum majorana essential oil ( OmEO ), and its various knock‐down effects against the rice moth, Corcyra cephalonica . GC–MS analysis detected the existence of major compounds such as monoterpenes, cis‐β‐terpineol and terpinen‐4‐ol with the total proportion of 52.16%. Fumigant toxicity against adult and larvae was calculated with an LC 50 value of 11.31 and 49.83 μL/L air, respectively. The contact toxicity against adult, pupa, larvae and eggs was observed with LC 50 value 2.54, 0.95, 2.78, and 0.49 μL/L, respectively. Furthermore, the influential repellent behavior against adults has been observed. Acetylesterase ( AChE ) inhibition activity of OmEO was observed against adult and larvae of C. cephalonica with an IC 50 value of 35.89 and 118.54 μL/mL, respectively. Moreover, computational docking study revealed the binding affinity of Cis‐β‐terpineol and terpinen‐4‐ol towards the active binding sites of AChE. On the other hand, Fluorescence‐assisted cytometry and comet assay confirmed the cytotoxic and genotoxic effect of OmEO at various concentrations on C. cephalonica . Altogether, the results showed the knock‐down effect of OmEO against C. cephalonica , and it could be a potential biocontrol measure against the stored product pest.