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Identification and characterization of heat shock proteins in a parasitic wasp Chouioia cuneae (Hymenoptera: Eulophidae)
Author(s) -
Pan LiNa,
Wang FengZhu,
Zhang XinYue,
Zhao YanNi,
Zhu GengPing,
Li Min
Publication year - 2018
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12251
Subject(s) - biology , eulophidae , hyphantria , abiotic component , heat shock protein , parasitoid , hymenoptera , pest analysis , real time polymerase chain reaction , botany , lepidoptera genitalia , ecology , gene , genetics
Heat shock proteins (HSPs) are known to be induced in response to various stress factors. Although HSPs have been studied in a number of insects, not much is known about HSPs in the natural enemies of insects, especially parasitoids. In this study, we identified and characterized five full‐length HSP genes ( Cchsp 40, Cchsp 60, Cchsp 70, Cchsp 83, and Cchsp 90) from an endoparasitic chalcid wasp, Chouioia cunea , which parasitizes the fall webworm, Hyphantria cunea pupae, a worldwide pest. The expression of Cchsps in response to temperature, pesticide stresses and UV radiation were also investigated by quantitative real‐time polymerase chain reaction (RT‐qPCR). The results showed that all five Cchsps were induced in response to hot and cold temperatures. Four pesticides induced the abundant expression of Cchsp 70, Cchsp 83 and Cchsp 90 while ultraviolet radiation up‐regulated Cchsp 40, Cchsp 70, Cchsp 83 and Cchsp 90. These results indicate the different transcriptional profiles of the five different Cchsps in response to various abiotic stresses. The findings of this study provide insights into the response of C. cunea to abiotic stresses and insight into the use of this parasitoid in biological control strategies.