Premium
Biochemical screening of Lactobacilli in midgut of wild Aedes aegypti mosquitoes. A step toward paratransgenesis candidate
Author(s) -
Maimusa Alhaji Hamisu,
Ahmad Abu Hassan,
Kassim Nur Faeza Abu,
Ahmad Hamdan,
Dieng Hamady,
Rahim Junaid,
Dahiru Muhammad Ahmad
Publication year - 2019
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12248
Subject(s) - midgut , aedes aegypti , biology , microbiology and biotechnology , bacteria , larva , gram staining , lactobacillus casei , aedes , bacillus thuringiensis , botany , genetics , antibiotics
This study was conducted to isolate and identify lactobacilli from larval and adult midgut of wild Aedes aegypti ( Ae. aegypti ) to find candidate bacteria for paratransgenic control. Characterization of the bacterial symbionts was done using Gram staining, motility test, catalase test, and biochemical tests, among others, and the morphological features were confirmed using a standard scheme that simplifies the identification of lactic acid bacteria. A total of 174 strains were isolated and identified, 135 strains from larval midgut and 39 strains from adult midgut (mean ± SE, 4.00 ± 0.72; P = 0.00). The isolated species were confirmed to be Lactobacillus fermentum , L. casei , L. acidophilus , L. viridescens , L. brevis and L. gasseri . It can be concluded that Ae. aegypti has the potential of harboring the cultivable bacterial symbionts. In conclusion, the isolated species were nominated for paratransgenic control, particularly L. fermentum , being found in large numbers from both larval and adulxt midgut.