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DNA data and morphology reveal the existence of Kentrochrysalis streckeri Staudinger, 1880 (Lepidoptera: Sphingidae) instead of K . consimilis in South Korea
Author(s) -
Kim Min Jee,
Kim SungSoo,
Choi SeiWoong,
Cho Youngho,
Kim Iksoo
Publication year - 2016
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12167
Subject(s) - biology , phylogenetic tree , dna barcoding , lepidoptera genitalia , morphology (biology) , divergence (linguistics) , barcode , evolutionary biology , zoology , botany , genetics , gene , linguistics , philosophy , computer science , operating system
In the present study, we determined that specimens of Kentrochrysalis consimilis collected from South Korea were K . streckeri , rather than K . consimilis , based on morphology, DNA barcodes and nuclear elongation factor 1 alpha ( EF‐1α ) sequences. The major morphological differences between K . streckeri and K . consimilis include the shape of forewing and hind‐wing pattern elements and male and female genitalia. The DNA barcode analysis of the South Korean specimens and the Russia‐originated K . streckeri showed a maximum sequence divergence of only 0.659% (4 bp), whereas that of the South Korean specimens and Japan‐originated K . consimilis showed a minimum sequence divergence of 2.965% (18 bp), indicating that the Korean specimens are, in fact, K . streckeri and not K . consimilis . Phylogenetic analyses both by Bayesian inference and maximum likelihood methods strongly clustered the South Korean specimens and Russian K . streckeri into one group, excluding K . consimilis . The EF‐1α ‐based sequence and phylogenetic analyses of the two species also supported data from the DNA barcode, indicating the distribution of K . streckeri in South Korea, instead of K . consimilis .