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Effect of recombinant baculovirus expressing C r V 1 protein from C otesia rubecula bracovirus against P ieris rapae in insecticidal toxicity
Author(s) -
Wei Lihua,
PerezRodriguez Miguel A.,
RodriguezPerez Mario A.
Publication year - 2016
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12160
Subject(s) - biology , trichoplusia , gene , recombinant dna , microbiology and biotechnology , baculoviridae , cabbage looper , autographa californica , gene expression , rna interference , virology , larva , rna , botany , genetics , spodoptera , noctuidae
Baculoviruses can be genetically engineered to express foreign genes; thus, their lethal potency and host range can be improved to produce more virulent bioinsecticides. Polydnavirus ( PDV ) genes have insecticidal bioactivities and could enhance the pathogenicity of the baculoviruses to control insect pests. The C r V 1 gene from C otesia rubecula polydnavirus is responsible for depolymerization of actin cytoskeleton in hemocytes, disabling its spread on foreign object surfaces. In this study, we tested the efficacy of the recombinant baculovirus ( A c MNPV ‐ C r V 1) under p10 promoter against second instar P . rapae larvae. The expression of the C r V 1 gene in P . rapae larvae was verified with reverse transcription–polymerase chain reaction ( RT‐PCR ). A c MNPV ‐ C r V 1 showed a significantly lower median lethal concentration ( LC 50 ) and shorter median lethal time ( LT 50 ) as compared with the A c MNPV wild‐type virus. These results suggested that the expression of C r V 1 protein could successfully improve the insecticidal toxicity of baculovirus.