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Differential induction of heat shock protein genes to the combined treatments of heat with diatomaceous earth, phosphine or carbon dioxide on P lodia interpunctella
Author(s) -
Kim Hanna,
Yu Yeon Su,
Lee KyeongYeoll
Publication year - 2015
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12139
Subject(s) - heat shock protein , hsp70 , biology , larva , carbon dioxide , shock (circulatory) , heat shock , heat stress , pest analysis , zoology , toxicology , gene , botany , biochemistry , ecology , medicine
Heating is one of the many disinfestation methods commonly used in facilities that store and process agricultural products. In this study, we have investigated whether the combination of heat treatment with diatomaceous earth ( DE ), phosphine ( PH 3 ) or CO 2 affects the mortality of the wandering larvae of P lodia interpunctella , which is a major pest found in most stored agricultural products. The mortality rate was 35.0% at day 1 after heat treatment at 40° C for 6 h; however, mortality rates increased after combined treatments of heat and 1 ppm DE or 10 ppm PH 3 , while 10% CO 2 had no significant effect. Quantitative real‐time PCR analysis showed that combined treatments involving 1 h of heat treatment with either DE , PH 3 or CO 2 increased the m RNA levels of four heat shock protein ( hsp ) genes ( hsp25 , hsp70 , grp78 and hsp90 ) in wandering larvae, 1 h post‐treatment, although those rates were slightly differentiated in each heat shock protein. Our results demonstrate that combinations of heat and DE or PH 3 show increased lethality, although insects produce stress responses at the molecular level.