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Temporal analysis of 16 STR loci in human blood drawn from two culicid mosquitoes cultured at different temperatures
Author(s) -
Ibrahim Ahmed M.A.,
Alrakan Lamia A.,
Alaifan Saleh Ahmed,
AlMekhlafi Fahd A.,
Kassab Ahmed Ch.
Publication year - 2015
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12123
Subject(s) - biology , genotyping , human blood , aedes aegypti , zoology , forensic entomology , microsatellite , veterinary medicine , blood meal , genotype , ecology , larva , genetics , gene , physiology , medicine , allele
Female mosquitoes feed on human blood, which can be collected to analyze human short tandem repeat ( STR ) sequences; these are specific to each human individual. Analysis of STR s might help in identification of a person found near a crime scene. A edes aegypti and C ulex pipiens mosquitoes fed on human blood were cultured at 18° C or 40° C (median temperature for summer and winter time in Riyadh governorate, S audi A rabia) for 3, 6, 12, 24, 48 and 72 h. In A . aegypti , human DNA concentration was reduced with time at both temperatures. At 18° C , we obtained full STR profiles up to 48 h post feeding on human blood while none of the 16 loci were obtained at 72 h. At 40° C , we missed six sites at 12 h after blood sucking, 12 at 24 h, and 15 at 48 h and 72 h. In C . pipiens cultured at 18° C , full profiles were developed up to 48 h following blood feeding while we could not amplify five sites at 72 h. At 40° C , mortality among females was 50% at 24 h and 100% at both 48 h and 72 h; however, we had full profiles in all samples including dead insects. This research addressed the possibility of using mosquitoes in forensic research by DNA genotyping by changing the mosquito culturing temperature and mosquito genus. Our findings proved that different types of mosquito change the temporal pattern of STR analysis and showed that the mosquito culturing temperature affects the integrity of DNA for STR analysis.