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Functionality of insect‐cell‐derived colorectal cancer vaccine candidate protein E p CAM‐F c in human dendritic cells
Author(s) -
Park Yo Seph,
Lee JeongHwan,
Park JinYeon,
Hwang Han Sung,
Zenke Martin,
Han Dong Wook,
Kang Young Sun,
Ko Kisung,
Ko Kinarm
Publication year - 2015
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12109
Subject(s) - biology , dendritic cell , immune system , recombinant dna , colorectal cancer , cancer immunotherapy , immunotherapy , immunology , cancer , microbiology and biotechnology , cancer research , gene , biochemistry , genetics
The baculovirus–insect cell expression system is widely used to produce recombinant proteins for various biomedical applications. Our previous study demonstrated that E p CAM , a colorectal cancer vaccine candidate protein, can be expressed in the baculovirus–insect cell expression system. However, its functionality (the ability to elicit an immune response), which is important for its possible use as a colorectal cancer vaccine for immunotherapy, still needed to be confirmed. In this study, we examined the ability of recombinant E p CAM to induce maturation of immature dendritic cells ( DC s) derived from CD 34 + cells isolated from human umbilical cord blood. We demonstrated that E p CAM induces the expression of four DC maturation markers: CD 80, CD 83, CD 86 and MHC II . These results suggest that E p CAM produced in the baculovirus–insect cell expression system is functional in terms of its ability to trigger maturation of human DC s.