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Silencing of apolipophorin‐ III causes abnormal adult morphological phenotype and susceptibility to L isteria monocytogenes infection in T enebrio molitor
Author(s) -
Patnaik Bharat Bhusan,
Patnaik Hongray Howrelia,
Park Ki Beom,
Jo Yong Hun,
Lee Yong Seok,
Han Yeon Soo
Publication year - 2015
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12099
Subject(s) - biology , rna interference , larva , hemolymph , gene silencing , rna silencing , phenotype , listeria monocytogenes , rna , microbiology and biotechnology , immunity , innate immune system , immunology , immune system , biochemistry , botany , gene , genetics , bacteria
Insect apolipophorin‐ III is an exchangeable protein that is abundantly found in the hemolymph, and serves an important role in lipid transport, development, and innate immunity. In this study, we examined the role of apolipophorin‐ III ( TmapoLp ‐ III ) during the adult eclosion stages of T enebrio molitor by RNA interference ( RNA i) analysis. After silencing of the m RNA transcripts at both larval and pupal stages, adult phenotypic defects were noticed. Defects included the incomplete shedding of pupal skin, shorter extension of the elytra, and improper folding of the hind wings. Most of the adults were malformed and died possibly due to dehydration. We also showed the involvement of TmapoLp ‐ III in conferring resistance to T . molitor larvae against L isteria monocytogenes infection. Mortality was found to be lower in non‐silenced intoxicated larvae while the TmapoLp ‐ III silenced larvae showed a significant susceptibility after 7 days post‐injection with a dose of 10 6 cfu/larvae.