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Molecular identification and phylogenetic analysis of economically important acaroid mites ( A cari: A stigmata: A caroidea) in K orea
Author(s) -
Khaing Tin Moe,
Shim JaeKyoung,
Lee KyeongYeoll
Publication year - 2014
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12085
Subject(s) - biology , phylogenetic tree , genbank , phylogenetics , ribosomal rna , internal transcribed spacer , botany , genetics , zoology , gene
Molecular diagnosis is highly valuable for the species identification of microscopic mites. Here, we collected some economically important mites of the superfamily A caroidea from various stored products in K orea. Those nucleotide sequences of ribosomal second internal transcribed spacer ( ITS2 ) and the mitochondrial cytochrome oxidase subunit I ( COI ) regions were determined by PCR using universial primers. In nucleotide sequence comparison at GenBank database, seven species including R hizoglyphus robini , R . echinopus , S ancassania sp. A carus siro , T yrophagus putrescentiae , T . similis in A caridae and S uidasia medanensis in S uidasiidae were identified. Particularly, COI sequences of R . robini , R . echinopus, S ancassania sp. and T . similis were firstly determined. Our results suggest that the phylogenetic relationships inferred from the ITS2 region, rather than COI region, were similar to those derived based on their morphological classification. Our study provides molecular information for the identification and phylogenetic relationship of acaroid mites in K orea.