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Partial sequence and characterization of nitric oxide synthase gene from H yphantria cunea
Author(s) -
Kim Hong Ja,
Kim Yong Il,
Kwon Yong Min,
Kang Young Jin,
Cheon Hyang Mi,
Ha Nam Gyu,
Seo Sook Jae
Publication year - 2013
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12040
Subject(s) - nitric oxide synthase , biology , nitric oxide , complementary dna , heme , atp synthase , biochemistry , gene , microbiology and biotechnology , nos1 , peptide sequence , enzyme , endocrinology
Nitric oxide synthase ( NOS ) gene has been partially sequenced from H yphantria cunea and compared with those already determined from insects. H yphantria cunea NOS possesses putative recognition sites for co‐factors heme, BH 4 , CaM , FMN , FAD , and NADPH common to NOS . The deduced amino acid sequence of H . cunea NOS cDNA showed 70.3% identity to M anduca sexta NOS and 57.6–69.5% identity to NOS sequences from other insects. Nitric oxide synthase is expressed in all tissues of H . cunea, except in hemocytes. The NOS expression in midgut, fat body, epidermis, and M alpighian tubule strongly increased against G ram‐positive and G ram‐negative bacterial infection. These results suggest that NOS may play an important role in insect defense system against bacterial infection.