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Genetic relationships between O eneis urda and O . mongolica ( N ymphalidae: L epidoptera)
Author(s) -
Kim SungSoo,
Wan Xinlong,
Kim Min Jee,
Kim Iksoo
Publication year - 2013
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/1748-5967.12013
Subject(s) - biology , phylogenetic tree , gene , population , genetic distance , genetics , internal transcribed spacer , mitochondrial dna , genetic divergence , evolutionary biology , genetic variation , genetic diversity , demography , sociology
Abstract The species status of O eneis urda ( E versmann) and O . mongolica ( O berthür) has been argued based on morphological characters. Reexamination of their major morphological characters has shown a slight differentiation in the two species. Sequences of three mitochondrial genes ( COI , ND 6, and ND 1) and one nuclear region (internal transcribed spacer 2, ITS 2) from two O . urda populations ( Y angyang and M t. H anla) and one O . mongolica population ( U ljin) were performed for phylogenetic and population genetic inferences. Sharing of identical sequences in the ND 6 gene and ITS 2, minimal sequence divergence in the COI and ND 1 genes, and phylogenetically undividable sequence types in all mitochondrial genes and ITS 2 suggest genetic continuity between the two species. Nevertheless, significant F ST estimates ( P < 0.05) were found for the COI gene in comparisons between Y angyang ( O . urda ) and U ljin ( O . mongolica ), between Y angyang ( O . urda ) and M t. H anla ( O . urda ), and between U ljin ( O . mongolica ) and M t. H anla ( O . urda ) populations. These F ST estimates, along with other gene‐based analyses collectively suggest isolation of the two species at some point in the past, but incomplete separation between the two species on the mainland ( Y angyang and U ljin) and biogeographically forced isolation of the O . urda population on M t. H anla collectively appear to complicate species status of these two species that were once further clearly separated.