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Adsorption kinetics of high mobility group box 1 protein in a polyacrylonitrile hemofiltration membrane
Author(s) -
Nakamura Tomoyuki,
Moriyama Kazuhiro,
Shimomura Yasuyo,
Kato Yu,
Kuriyama Naohide,
Hara Yoshitaka,
Yamada Shingo,
Nishida Osamu
Publication year - 2021
Publication title -
therapeutic apheresis and dialysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.415
H-Index - 53
eISSN - 1744-9987
pISSN - 1744-9979
DOI - 10.1111/1744-9987.13489
Subject(s) - membrane , adsorption , hmgb1 , hemofiltration , polyacrylonitrile , medicine , membrane fouling , chromatography , fouling , chemical engineering , chemistry , polymer , surgery , biochemistry , organic chemistry , inflammation , hemodialysis , engineering
The high mobility group box 1 protein (HMGB1) is recognized as a prototypical endogenous danger cytokine in sepsis. We previously reported that a polyacrylonitrile (AN69ST) membrane rapidly adsorbed HMGB1. Herein, an in vitro hemofiltration system was designed to assess the HMGB1 adsorption capacity, adsorption sites, and adsorption mechanism of the AN69ST membrane. HMGB1 was repeatedly added seven times during hemofiltration. A rapid decrease in circulating HMGB1 was observed after every addition with no sign of saturation. Presence of HMGB1 on the filter membrane was observed on both membrane surfaces and within the bulk layer using a high concentration of HMGB1 by immunoelectron microscopy. We hypothesized that the addition of heparin to the membrane surface or filtration rate would contribute to the adsorption mechanism. We could not measure the influence of heparin and filtration. Although the membrane was too large to saturate under the μg/mL HMGB1 conditions, our results show that the AN69ST membrane has a robust absorption capacity that could be used to treat sepsis.